Microbiome of the Uterus in Women With Unsuccessful in Vitro Fertilization Attempts
| Autor: | Bushtyreva Irina Olegovna, Barinova Viktoriya Vladislavovna, Aseev Mihail Vladimirovich, Polev Dmitry Evgenievich, Dudurich Vasilisa Valerievna, Kuznetsova Natalya Borisovna, Sokolova Kristina Mikhailovna |
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| Rok vydání: | 2020 |
| Předmět: |
Infertility
030219 obstetrics & reproductive medicine In vitro fertilisation biology business.industry medicine.medical_treatment Uterus Obstetrics and Gynecology Physiology Urine biology.organism_classification medicine.disease 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Reproductive Medicine Lactobacillus medicine 030212 general & internal medicine Microbiome business Luteinizing hormone Uterine microbiome |
| Zdroj: | International Journal of Women's Health and Reproduction Sciences. 8:423-427 |
| ISSN: | 2330-4456 |
| DOI: | 10.15296/ijwhr.2020.68 |
| Popis: | Objectives: This study aimed to investigate the peculiarities of the uterine microbiome in the case of infertility and repeated in vitro fertilization (IVF) failures. Accordingly, the microbiome of the uteri of 22 women with 2 or more IVF failures (first group) and 20 healthy women (second group) was analyzed in this study. Materials and Methods: The samples of uterine microbiomes were collected 7 days after the luteinizing hormone elevation, which was determined by the urine test. All measures were taken to avoid sample contamination. Finally, the massively parallel sequencing of the 16S ribosomal RNA gene was done in every uterine sample. Results: The analysis of the mean relative abundance of various microorganisms in the uterine microbiome showed that women with infertility had higher microbiological diversity and variability compared to healthy women. Eventually, the mean relative abundance of the Lactobacillus genus comprised 34.4% and 63.0% in the first and second groups, respectively. Conclusion: In general, the presence of Lactobacillus in the uterine microbiome could be considered a favorable factor for good reproductive outcomes and successful IVF programs. |
| Databáze: | OpenAIRE |
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