Probing conformational changes with multiwavelength collisional quenching (769.6)

Autor: Emily Bartle, Chun Li, Philip Varnes, Ellis Bell, Carlos Metz, Forest Barkdoll-Weil
Rok vydání: 2014
Předmět:
Zdroj: The FASEB Journal. 28
ISSN: 1530-6860
0892-6638
DOI: 10.1096/fasebj.28.1_supplement.769.6
Popis: Collisional quenching approaches using neutral or charged quencher molecules has long been used to indicate conformational changes in proteins Here we introduce a novel approach to collisional quenching using multiwavelength analysis of spectral data collected at two different excitation wavelengths with a variety of small molecule and protein test fluorophores. The results show that not only can detailed information about the local environment of tryptophans in proteins be obtained (even proteins with multiple tryptophans), but information is derived resulting from tyrosine-tryptophan resonance energy transfer in proteins... Using Acrylamide, Iodide and Cesium collisional quenchers with both tyrosine and tryptophan derivatives and with model proteins with one or more tryptophans we demonstrate the utility of the approach for defining the local environment and changes induced by ligand binding, Furthermore, during the course of these studies we revealed that pi-cation effects disrupt resonance energy tran...
Databáze: OpenAIRE
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