Cultured and freshly isolated adipose tissue-derived cells: fat years for cardiac stem cell therapy

Autor: Pedro L. Sánchez, Ma Eugenia Fernandez-Santos, Francisco Fernández-Avilés, Ricardo Sanz-Ruiz
Rok vydání: 2009
Předmět:
Zdroj: European Heart Journal. 31:394-397
ISSN: 1522-9645
0195-668X
DOI: 10.1093/eurheartj/ehp403
Popis: As understanding has grown about what happens when heart muscle cells die en masse after a heart attack, so has the development of treatments aimed at avoiding the loss or failure of cardiovascular function. These treatments include stem cell transplantation. The first pre-clinical and clinical experiences in stem cell transplantation were reported around a decade ago.1,2 However, and although the field has moved quickly in these years, the most important questions—the ‘cell product’ to be used, delivery method, outcome measurement, organizational problems, and funding—remain unanswered. The quest for the best cell type has been wheeled around from bedside to bench and back again.3 The ideal cell type should be capable of differentiating into functional cardiomyocytes and forming new vessels. Cell types from several different sources have already been tested in animal models, and bone marrow-derived cells and skeletal myoblasts have already been used in clinical trials. Each subtype has its advantages and disadvantages, but one important issue is the time needed for their preparation: time-consuming protocols hinder easy implementation in the acute clinical setting and carry a risk of culture contamination and/or genetic modification. Furthermore, whether the beneficial effect is mediated by one specific cell type or many cell types in concert remains unknown. It is obviously of great scientific interest to test all possible combinations of progenitor types, numbers of cells, times of injection after damage, frequencies of injection, mechanisms of action, as well as homing/grafting and survival, all through different routes in animal models and then in man. The report by Bai et al. 4 is significant for different reasons. First, it demonstrates that freshly isolated adipose tissue-derived stem cells (ASCs) transplanted into ischaemic hearts after acute myocardial infarction promote cardiac function as well as do cultured ASCs. Secondly, it indicates that transplanted cells … *Corresponding author. Tel: +34 91 426 5880, Fax: +34 91 586 8276, Email: faviles{at}secardiologia.es
Databáze: OpenAIRE
Externí odkaz: