Massively parallel sequencing for the forensic scientist – sequencing archived amplified products of AmpFlSTR Identifiler and PowerPlex Y multiplex kits to capture additional information

Autor:	Ryan England, SallyAnn Harbison
Rok vydání:	2016
Předmět:	0301 basic medicine Genetics Massive parallel sequencing Illumina miseq Computational biology Biology Amplicon DNA sequencing Pathology and Forensic Medicine Forensic science 03 medical and health sciences 030104 developmental biology Microsatellite Multiplex Sequence variation
Zdroj:	Australian Journal of Forensic Sciences. 49:308-325
ISSN:	1834-562X 0045-0618
DOI:	10.1080/00450618.2015.1134658
Popis:	In the last few years the cost and ease of massively parallel sequencing (MPS) has reduced dramatically to the point that it can now be considered as a tool for use in forensic case work. An important consideration for the implementation of any new forensic technology is the ability to remain compatible with previous technology. During this study we sequenced the amplicons of two commercial forensic short tandem repeat (STR) multiplexes AmpFlSTR Identifiler and PowerPlex Y using the Illumina MiSeq and Ion PGM Sequencer (Life Technologies) and characterised the sequence data from a forensic perspective. Using the MPS data from both platforms we determined the STR genotypes of forensic samples and found previously undocumented sequence variation in seven STR alleles. By characterising features of the DNA sequence profiles, such as stutter and locus imbalance we identified areas for future development that will be needed prior to casework implementation. The rapid development of this technology has meant man...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a0360d1884a060f174cb8408adfefc83 https://doi.org/10.1080/00450618.2015.1134658 Zobrazit plný text záznamu