Gastric acid secretion in cyclooxygenase-1 deficient mice

Autor: Ingvar Bjarnason, Gudmunder Sigthorsson, Iggy Tavares, N J Welsh, A Palizban, F Borrelli, Robert J. Simpson
Rok vydání: 2000
Předmět:
Zdroj: Alimentary Pharmacology & Therapeutics. 14:1365-1370
ISSN: 0269-2813
DOI: 10.1046/j.1365-2036.2000.00836.x
Popis: Background: Constitutive cyclooxygenase-1 enzyme synthesizes prostaglandins which are thought to play an important role in the functional integrity of the stomach gastric mucosa. Recently, it was shown that cyclooxygenase-1 deficient mutant mice did not develop spontaneous gastric pathology and appear less sensitive to indomethacin-induced gastric damage. Aim: To investigate gastric acid secretion in cyclooxygenase-1 deficient mutant mice. Methods: The basal and histamine or isobutyl methylxanthine-stimulated acid secretion in stomachs of cyclooxygenase-1 deficient homozygous mice and the effect of indomethacin was compared with that of heterozygous and wild-type mice using isolated lumen perfused mouse stomachs, in organ baths, monitored by pH-electrodes. Results: There was no significant difference in the basal or histamine stimulated gastric acid secretion between wild-type or heterozygous or homozygous mice. However, isobutyl methylxanthine was more potent in the cyclooxygenase-1 deficient and heterozygous mice than in wild-type mice. Indomethacin, at concentrations below 1 mM, had no effect on either basal or histamine stimulated acid secretion in any of the mice populations. Conclusion: Gastric acid secretion is maintained without prostaglandin involvement in cyclooxygenase-1 deficient mice. The finding that basal and histamine-stimulated gastric acid secretion was similar in the cyclooxygenase-1 deficient, compared to wild-type mice is consistent with the lack of spontaneous gastric pathology in the cyclooxygenase-1 deficient mice.
Databáze: OpenAIRE
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