Hydrophobic PDMS promotes neural progenitor formation from SHEDs by Schwann cell–cultivated medium induction
| Autor: | Wen-Ta Su, Yu-Ching Huang, Yu-Jing Pan, Te-Yang Huang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Glial fibrillary acidic protein biology Chemistry General Chemical Engineering Schwann cell Nestin Analytical Chemistry Cell biology Reverse transcription polymerase chain reaction 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Tubulin Neurosphere Gene expression biology.protein medicine Stem cell 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Polymeric Materials and Polymeric Biomaterials. 67:11-19 |
| ISSN: | 1563-535X 0091-4037 |
| DOI: | 10.1080/00914037.2017.1297937 |
| Popis: | In this study, the ability of rat Schwann cell-conditioned medium to transdifferentiate stem cells from human exfoliated deciduous teeth (SHEDs) into neural-like cells was evaluated. Furthermore, the potential effects of the hydrophobic polymer polydimethylsiloxane on neurosphere formation by the neural-like transdifferentiated SHED were investigated. The inducing effects of Rat Schwann cell-cultivated medium (RSC-CM) to SHED differentiation were investigated by inverted phase contrast microscopy. Cellular clusters formed fast at 7-day culture. Immune-fluorescent staining and neural gene expression performed by reverse transcription polymerase chain reaction showed the positive expression of nestin, tubulin III, γ-enolase, and glial fibrillary acidic protein of neural marker. Thus, RSC-CM can induce effectively neural differentiation from SHED cells. |
| Databáze: | OpenAIRE |
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