High rate of restriction fragment length polymorphisms between two population of the nematode Pristionchus pacificus (Diplogastridae)
| Autor: | Ralf J. Sommer, Andreas Eizinger, Isabel Schlak |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Genetics
education.field_of_study biology ved/biology ved/biology.organism_classification_rank.species Population EcoRI biology.organism_classification Caenorhabditis genomic DNA Pristionchus pacificus Genetic model biology.protein Coding region Animal Science and Zoology Restriction fragment length polymorphism education Molecular Biology Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Zoological Systematics and Evolutionary Research. 35:137-142 |
| ISSN: | 1439-0469 0947-5745 |
| DOI: | 10.1111/j.1439-0469.1997.tb00415.x |
| Popis: | Pristionchus pacificus (Diplogastridae, Nematoda) has recently been described as a ‘satelite’ organism for a functinal comparative approach, becuase genetic, molecular and cell-biological tools can be used in way similar to the genetic model organism Caenorhabditis elegans. This tudy describes the analysis of two previously isolated strains of P. pacificus for the occurrence of restriction fragment length polymorphisms (RFLPs). In all, 14 of 17 randomly chosen cDNA clones give polymorphisms after hybridization to EcoRI digested genomic DNA of the populations from California and Washington. This polymorphism is much higher than polymorphism found among different strains in C. elegans. Therefore this study compares most of the nucleotide sequence of the Ppa-let-60/ras gene between the two strains. No base-pair substitutions were found between these two sequences within the coding regions. However, within the untranslated region, four base-pair substitutions in introns and the deletion of three base-pairs in the 5′ sequence and in intron 4 have been observed. Since the two strains interbreed, RFLPs can be used as molecular markers for future chromosomal walking and gene cloning. |
| Databáze: | OpenAIRE |
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