| Autor: |
R W Jeanloz, Geneviève Lamblin, Nasir-ud-Din, M Lhermitte |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Journal of Biological Chemistry. 260:9981-9987 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(17)39199-8 |
| Popis: |
The external polysaccharide is a major component of Micrococcus lysodeikticus cell wall and displays distinct composition. The complete structure of the external polysaccharide had been elucidated as a basis for investigation of the cell wall structure-function relation. However, the mode of attachment of the polysaccharide to the peptidoglycan through a phosphodiester was not clear due to limitations in structural and biosynthetic studies. The present study describes purification of a lysozyme-resistant nondialyzable high-molecular-weight fragment of cell wall and identifies the sugar, D-glucose, as the point of external polysaccharide attachment to the peptidoglycan through a phosphate diester. Kinetic studies for the acid-catalyzed release of external polysaccharide from the peptidoglycan were performed in parallel with synthetic [methyl-2-acetamido-3-O-(D-1-carboxyethyl)-2-deoxy-alpha-D- glucopyranoside-6-yl]-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranosyl phosphate and showed the presence of a phosphodiester linkage between external polysaccharide and peptidoglycan. In addition, type of phosphate residue and cross-linking between muramic acid and protein part have been determined. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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