Immunohistochemical Localization of Manserin, a Novel Neuropeptide Derived from Secretogranin II, in Rat Adrenal Gland, and its Upregulation by Physical Stress
| Autor: | Naoko Narita, Yoshihiro Komada, Masaaki Narita, Naoki Kamada, Yoshio Imura, Kaori Tano, Atsuko Uchida, Yasura Tashiro, Akiko Oyabu |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Chemistry Colocalization Neuropeptide Bioengineering Biochemistry Analytical Chemistry Phenylethanolamine chemistry.chemical_compound medicine.anatomical_structure Endocrinology Downregulation and upregulation Cortex (anatomy) Internal medicine Drug Discovery medicine Molecular Medicine Endocrine system Immunohistochemistry Adrenal medulla |
| Zdroj: | International Journal of Peptide Research and Therapeutics. 16:55-61 |
| ISSN: | 1573-3904 1573-3149 |
| DOI: | 10.1007/s10989-010-9203-2 |
| Popis: | We recently identified a novel 40-amino acid neuropeptide designated manserin from the rat brain (Yajima in NeuroReport 15: 1755–1759, 2004). Manserin is highly expressed in pituitary and hypothalamic nuclei, which suggests that it plays a role in the endocrine system. In this study, we employed immunohistochemical methods to investigate the presence of manserin in rat adrenal glands, as well as its regulation by physical stress. Immunohistochemical analysis using anti-manserin antibody showed that manserin is present in the rat adrenal medulla but not in the cortex. When the colocalization of manserin and phenylethanolamine N-methyltransferase (PNMT), an epinephrine-synthesizing enzyme, was examined, virtually all PNMT-positive cells expressed manserin. Interestingly, the immunoreactivity of manserin was significantly increased when the rats were exposed to water-immersion restraint stress. These results demonstrate for the first time that adrenal manserin, a novel neuropeptide, may have a potential physiological role under stress-inducing conditions. |
| Databáze: | OpenAIRE |
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