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Background Epithelial-mesenchymal transition (EMT) plays a major role in tumor invasion and metastasis, which is associated with poor survival in surgically resected lung cancer. Tumor microenvironment, especially extracellular acidity is a favorable factor for tumor progression and metastatic spread, and participates in EMT. However, the effects of Hypercapnic acidosis (HCA) microenvironment on EMT of lung cancer still unknown.Materials and Methods The acid-sensing ion channels (ASICs) gene expression and prognostic value in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) were evaluated using online database. Human lung adenocarcinoma A549 cells were cultured in 10% CO2 and 90% air for 48h to assess the effects of the HCA microenvironment. Transwell assay and wound-healing assay of A549 cells were performed to determine the effects on EMT.Results The mRNA expression levels of ASIC3 may be useful for prediction of lung cancer patient overall survival. The expression of ASIC3 significantly increased , and the invasive and migrate ability of A549 cells was promoted after exposing in HCA microenvironment (10% CO2 and 90% air), and inhibited when ASIC3 was blocked.Conclusion HCA microenvironment induced EMT in lung cancer cells through ASIC3, which may have influence on the FP and OS of lung cancer patients. |
