Plant radioresistance and DNA repair efficiency inChlamydomonas reinhardtii andPisum sativum

Autor: A. D. Mehandjiev, S. A. Sergeeva, E. D. Blagoeva, S. N. Ptitsina, Stephka Chankova, V. A. Shevchenko
Rok vydání: 1994
Předmět:
Zdroj: Biologia plantarum. 36:583-589
ISSN: 1573-8264
0006-3134
Popis: This paper compares the repair of DNA single strand breaks (ssb) induced by γ-radiation in two strains ofChlamydomonas reinhardtii (137C/+/ and UVS-I) and three lines ofPisum sativum (NN 131, 198, 140) differing in the degree of radioresistance. DNA ssb in cells exposed to γ-rays (50, 100, 200, 500 Gy) were measured by electrophoresis and alkaline unwinding method with subsequent chromatography on hydroxyapatite immediately after irradiation and after 30 min of post-irradiation incubation at 25°C. An increase of double-strand DNA (in%) was found in cells after 30 min post-irradiation incubation.C. reinhardtii strains displayed an equal level of DNA degradation and repair efficiency in the DNA single strand breaks. The radioresistant line N 198 ofP. sativum is characterized by a lower level of induced DNA ssb and higher efficiency of repair of these breaks as compared with less radioresistant lines NN 131 and 140.
Databáze: OpenAIRE
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