Label-free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme

Autor:	Lun Wang, Wu Gong, Haiyan Wang, Zhian Tan, Xunxun Yin
Rok vydání:	2012
Předmět:	Chemistry General Chemical Engineering Aptamer Adenosine Small molecule Combinatorial chemistry chemistry.chemical_compound Biochemistry Colloidal gold Electrochemistry medicine Electrochemiluminescence Lysozyme Bifunctional DNA medicine.drug
Zdroj:	Electrochimica Acta. 76:416-423
ISSN:	0013-4686
DOI:	10.1016/j.electacta.2012.05.056
Popis:	A label-free bifunctional electrochemiluminescence (ECL) aptasensor for parallel detection of small molecule (adenosine) and protein (lysozyme) was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. Thiolated single-stranded DNA (DNA1) containing adenosine aptamer and sequence complementary to DNA2 was first immobilized on the Au electrode via sulfur–gold affinity, DNA2 containing lysozyme aptamer and sequence complememtary to DNA3 was bound to the electrode via forming DNA/DNA duplex. Gold nanoparticles functionalized with DNA3 (DNA3-GNPs) were used as signal amplifier to enhance the sensitivity of the aptasensor. With Ru(phen) 3 2+ as the signal transducer, the aptasensor exhibited high sensitivity and specificity. Under optimal conditions, a linear range from 0.5 to 7 nM for adenosine ( R = 0.999) and from 0.5 to 9 ng/mL for lysozyme ( R = 0.999) was obtained, respectively. The proposed method may provide a promising platform for ECL parallel detection of small molecules and protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9ff6912694dbef042ac57a6516a0175a https://doi.org/10.1016/j.electacta.2012.05.056 Zobrazit plný text záznamu Full Text from ScienceDirect