Gel Electrophoresis
| Autor: | A. Drabik, A. Bodzoń-Kułakowska, J. Silberring |
|---|---|
| Rok vydání: | 2016 |
| Předmět: | |
| DOI: | 10.1016/b978-0-444-63688-1.00007-0 |
| Popis: | Various types of electrophoretic separations are commonly used in proteomic approach. This chapter describes principle of electrophoresis in agarose, polyacrylamide, isoelectrofocusing, and 2-dimensional separations. Separations in native or denaturing conditions are discussed. This is followed by discussion of protein staining techniques for quantitation and visualization. Advantages and drawbacks are provided for the optimal choice of the most suitable method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|