BİOLOGİCAL AND PHYTOCHEMİCAL İNVESTİGATİONS OF EXTRACTS FROM PTEROCARPUS ERİNACEUS POİR (FABACEAE) ROOT BARKS
| Autor: | Hay Anne-Emmanuelle, Nikiema Jean-Baptiste, Lompo Marius, Guissou Innocent Pierre, Ouédraogo Jean Claude W, Tibiri André, Sawadogo Wamtinga Richard, Koudou Jean, Dijoux-Franca Marie-Genevieve, Ouedraogo Noufou |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Stigmasterol Traditional medicine Erinaceus biology Chemistry DPPH Friedelin biology.organism_classification 01 natural sciences Croton 0104 chemical sciences Lipid peroxidation 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Complementary and alternative medicine Biochemistry Phytochemical Drug Discovery Pterocarpus erinaceus |
| Zdroj: | African Journal of Traditional, Complementary and Alternative medicines. 14:187-195 |
| ISSN: | 2505-0044 0189-6016 |
| Popis: | Background: Pterocarpus erinaceus Poir. belonging to Fabacae familly is used as medicinal plant in Burkina Faso’s folk medicine. Roots of P. erinaceus are used to treat ulcer, stomach ache and inflammatory diseases. The objective of the present study was to carry out phytochemical composition of methanol (MeOH) and dichloromethane (DCM) extracts from Pterocarpus erinaceus roots, to isolate pure compounds, and to evaluate their pharmacological activities. Methods: Chromatographic fractionation led to the isolation of active components of the extracts. The structures were established by NMR analysis and comparison with data from literature. The anti-inflammatory activity was evaluated using croton oil-induced edema of mice ear as well as the effect of extracts against lipoxygenase and lipid peroxidation was evaluated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric-reducing antioxidant capacity (CUPRAC) methods were used to evaluate the antioxidant activity of the extracts. Results: Friedelin (1), 3α-hydroxyfriedelan-2-one (2), α-sophoradiol (3) and stigmasterol (4) were isolated from DCM extract and maltol-6-O-apiofuranoside-glucopyranoside (5) isolated from MeOH. DCM extract and friedelin, 3α-hydroxyfriedelan-2-one, α- sophoradiol showed a significant anti-inflammatory effect against ear edema. Friedelin (1), α-sophoradiol (3) and maltol-6-Oapiofuranoside- glucopyranoside (5) exhibited lipoxygenase inhibition. MeOH extract (100 μg/mL) inhibited lipoxygenase and lipid peroxidation activities at 45.1 ± 3% and 30.7 ± 0.5% respectively. MeOH extract, ethyl acetate fraction and butanol fraction exhibited antioxidant property with both two methods used. Conclusion: The results suggested that the extracts and compounds from roots of Pterocarpus erinaceus possessed local antiinflammatory effect, antioxidant properties and inhibitor effect against lipoxygenase and lipid peroxidation activities. |
| Databáze: | OpenAIRE |
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