Regeneration of plants from long-term root culture of lime, Citrus aurantifolia (Christm.) Swing

Autor:	K. P. S. Chandel, S. R. Bhat, P. Chitralekha
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Sucrose fungi Morphogenesis food and beverages Plant physiology Horticulture Biology biology.organism_classification Pericycle Tissue culture chemistry.chemical_compound Rutaceae chemistry Auxin Shoot Botany
Zdroj:	Plant Cell, Tissue and Organ Culture. 29:19-25
ISSN:	1573-5044 0167-6857
Popis:	Excised root culture of lime, C. aurantifolia, was established in Murashige & Skoog medium containing 3% sucrose. De novo shoot bud initiation was recorded in basal medium at a low frequency during three years of continuous culture. The effect of indole-3-acetic acid and benzyladenine (BA) were investigated on root growth and shoot morphogenesis. Inclusion of BA in the culture medium had an important effect on root growth and shoot elongation: the frequency of shoot regeneration was only slightly improved by the growth regulator treatment. Shoot bud differentiation occurred at the proximal region of the root at or just distal to the cut end. Histology of shoot regeneration revealed the endogenous origin of the shoot buds from the pericycle tissue of the root. The regenerated plants contained normal diploid chromosome number (2n=18) and have been established in soil.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9fd40bc26e3acb156407b6b594f567c7 https://doi.org/10.1007/bf00036141 Zobrazit plný text záznamu Full text from SpringerLink