Rheotaxis Based High-Throughput Motile Sperm Sorting Device
| Autor: | Byungkyu Kim, Dongkyu Lee, Bohyun Hwang, Seung-Jun Hwang, Joong-Hwan Baek |
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| Rok vydání: | 2019 |
| Předmět: |
0209 industrial biotechnology
Sperm sorting Chemistry Mechanical Engineering Sorting Motility Semen 02 engineering and technology Reproductive technology Sperm Industrial and Manufacturing Engineering 020303 mechanical engineering & transports 020901 industrial engineering & automation Human fertilization 0203 mechanical engineering Electrical and Electronic Engineering Sperm motility Biomedical engineering |
| Zdroj: | International Journal of Precision Engineering and Manufacturing. 20:1037-1045 |
| ISSN: | 2005-4602 2234-7593 |
| Popis: | A progressive motile sperm sorting device with a diffuser-type microfluidic channel is proposed to improve fertilization in assisted reproductive technologies. When sperm cells swim against a fluid flow, they are positioned according to their motility in specific channel zones. A numerical simulation is carried out in a meso-sized diffuser-type channel which can realize high throughput to verify the working principle and the sorting device is fabricated based on the simulation result. Furthermore, to analyze the condition of the semen and investigate the experimental results, a novel image processing method is proposed. With the proposed analyzing technique, the sperm sorting experiment is performed in the channel using the canine semen sample. As a result, the %motility increases from 59.89 to 69.36%, however, it does not satisfy the reported guideline for canine sperm motility (%motility > 70%). Therefore, we perform a washing process by injecting DPBS fluids to improve indexes related to motility. After double washing procedures, we could increase %motility from 59.89 to 82.24%, motile sperm rate from 33.38 to 53.10% and motility index from 26.35 to 49.44 in Zone A, comparing to the prepared sample. |
| Databáze: | OpenAIRE |
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