Isolation and expression analysis of three different flowering genes(TtLFY, TtAP1, and TtAP2) from an unusual legume species, Thermopsis turcica
| Autor: | Süleyman Cenkci, Mustafa Kargioğlu, Büşra Kahraman, Yaşar Karakurt, Alperen Dedeoğlu |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine fungi food and beverages Plant Science Biology biology.organism_classification 01 natural sciences Sepal Housekeeping gene Turcica 03 medical and health sciences 030104 developmental biology Arabidopsis Shoot Botany Petal Leafy Gene 010606 plant biology & botany |
| Zdroj: | TURKISH JOURNAL OF BOTANY. 40:447-460 |
| ISSN: | 1303-6106 1300-008X |
| DOI: | 10.3906/bot-1509-15 |
| Popis: | LEAFY (LFY), APETALA1 (AP1), and APETALA2 (AP2) genes encode three different transcription factors that control and regulate flower initiation and development in Arabidopsis. By using 3'- and 5'-RACE analysis, we isolated and sequentially characterized TtLFY (a LEAFY-like gene), TtAP1 (a MADS-box-like gene), and TtAP2 (an AP2/ERBF-like gene) in Thermopsis turcica, an unusual endemic legume species with three free carpellated flower structure. Semiquantitative RT-PCR analysis for 18 different vegetative and reproductive tissues of T. turcica indicated TtLFY transcripts mainly in the shoot tips and young floral buds and TtAP1 transcripts in the sepals and petals; however, TtAP2 transcripts were detected in all tissues. This is the first record for a LFY-like gene, TtLFY, expressed in the shoot tips of an underground plant section and for an AP2-like gene transcript found in all tissues, similar to a housekeeping gene. |
| Databáze: | OpenAIRE |
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