Імуногенетичні аспекти протизапальної терапії гострого обструктивного бронхіту в дітей раннього віку
| Autor: | O.Ye. Abaturov, O.O. Rusakova |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
biology medicine.diagnostic_test business.industry Total ige Inflammation Immunoglobulin E Gastroenterology Flow cytometry Proinflammatory cytokine Blood serum Internal medicine Immunoassay biology.protein medicine General Earth and Planetary Sciences Clinical efficacy medicine.symptom business General Environmental Science |
| Zdroj: | CHILD`S HEALTH. :41-45 |
| ISSN: | 2307-1168 2224-0551 |
| Popis: | The objective of the study was to improve the treatment of acute obstructive bronchitis in infants by optimizing the anti-inflammatory therapy based on the evaluation of its clinical, immunological and molecular genetic efficiency. Materials and methods. We have carried out a comprehensive examination of 80 children aged 6 months to 3 years with acute obstructive bronchitis. Patients were divided into two groups: children of the first group (n = 40) received systemic glucocorticosteroids, children of the second group (n = 40) were treated with inhaled glucocorticosteroids. Before and after the treatment, in all children we have studied the content of IFN-γ, IL-4 and IL-13 in the blood serum using enzyme linked immunosorbent assay, the concentration of total IgE — by means of electrochemiluminescent immunoassay. The level of expression of the transcription factor NF-κB in peripheral blood lymphocytes was determined using flow cytometry. Results. Transcription factor NF-κB, having almost the same effect on the concentration of IFN-γ and IgE in the blood serum, determines the characteristics of inflammation, mainly local, in acute obstructive bronchitis. Glucocorticosteroid therapy leads to the disappearance of NF-κB influence on the content of proinflammatory cytokines. Inhaled glucocorticosteroids, in addition, help to reduce the concentration of IgE in the blood serum and inhibition of the activity of pro-inflammatory intracellular cascades that, at high clinical efficacy and safety profile, justifies the appropriateness of their use in the treatment of acute obstructive bronchitis in infants as pathogenetic therapy. |
| Databáze: | OpenAIRE |
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