Role of the Nod Factor Hydrolase MtNFH1 in Regulating Nod Factor Levels during Rhizobial Infection and in Mature Nodules of Medicago truncatula
| Autor: | Kirankumar S. Mysore, Thomas Boller, Jie Cai, Wei Liu, Lan-Yue Zhang, Ye Tian, Christian Staehelin, Jiangqi Wen, Zhi-Ping Xie, Jin-Song Xiong, Yi-Han Wang, Hao-Ming Li, Ru-Jie Li |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sinorhizobium meliloti biology fungi food and beverages Cell Biology Plant Science Nod Root hair biology.organism_classification 01 natural sciences Medicago truncatula Microbiology Rhizobia Nod factor 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Symbiosis Chitin chemistry 010606 plant biology & botany |
| Zdroj: | The Plant Cell. 30:397-414 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.17.00420 |
| Popis: | Establishment of symbiosis between legumes and nitrogen-fixing rhizobia depends on bacterial Nod factors (NFs) that trigger symbiosis-related NF signaling in host plants. NFs are modified oligosaccharides of chitin with a fatty acid moiety. NFs can be cleaved and inactivated by host enzymes, such as MtNFH1 (MEDICAGO TRUNCATULA NOD FACTOR HYDROLASE 1). In contrast to related chitinases, MtNFH1 hydrolyzes neither chitin nor chitin fragments, indicating a high cleavage preference for NFs. Here, we provide evidence for a role of MtNFH1 in the symbiosis with Sinorhizobium meliloti. Upon rhizobial inoculation, MtNFH1 accumulated at the curled tip of root hairs, in the so-called infection chamber. Mutant analysis revealed that lack of MtNFH1 delayed rhizobial root hair infection, suggesting that excess amounts of NFs negatively affect the initiation of infection threads. MtNFH1 deficiency resulted in nodule hypertrophy and abnormal nodule branching of young nodules. Nodule branching was also stimulated in plants expressing MtNFH1 driven by a tandem CaMV 35S promoter and plants inoculated by a NF-overproducing S. meliloti strain. We suggest that fine-tuning of NF levels by MtNFH1 is necessary for optimal root hair infection as well as for NF-regulated growth of mature nodules. |
| Databáze: | OpenAIRE |
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