Molecular Characterization and Expression Analysis of S6K1 in Cashmere Goats ( Capra hircus )

Autor:	Wang Zhigang, Zheng Xu, Wu Manlin, Wang Yanfeng, Bao Wenlei, Hao Xiyan
Rok vydání:	2013
Předmět:	Cell growth P70-S6 Kinase 1 Biology Bioinformatics Molecular biology Ribosomal s6 kinase Open reading frame Complementary DNA Capra hircus biology.protein Animal Science and Zoology Eukaryotic Small Ribosomal Subunit Gene Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 26:1057-1064
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2012.12710
Popis:	p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession {"type":"entrez-nucleotide","attrs":{"text":"GU144017","term_id":"269204790","term_text":"GU144017"}}GU144017) and its 3′ noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3′ noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.
Databáze:	OpenAIRE
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