Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand

Autor:	Kanchana Rungsihirunrat, Kesara Na-Bangchang, Wanna Chaijaroenkul, Poonuch Muhamad, Jiraporn Kuesap
Rok vydání:	2015
Předmět:	medicine.medical_specialty biology Plasmodium vivax Endemic area Drug resistance biology.organism_classification medicine.disease Virology Dried blood spot Infectious Diseases Chloroquine Molecular genetics parasitic diseases medicine Parasitology Gene Malaria medicine.drug
Zdroj:	The Korean Journal of Parasitology. 53:43-49
ISSN:	1738-0006 0023-4001
DOI:	10.3347/kjp.2015.53.1.43
Popis:	The aim of the study was to explore the possible molecular markers of chloroquine resistance in Plasmodium vivax isolates in Thailand. A total of 30 P. vivax isolates were collected from a malaria endemic area along the Thai-Myanmar border in Mae Sot district of Thailand. Dried blood spot samples were collected for analysis of Pvmdr1 and Pvcrt-o polymorphisms. Blood samples (100 μl) were collected by finger-prick for in vitro chloroquine susceptibility testing by schizont maturation inhibition assay. Based on the cut-off IC50 of 100 nM, 19 (63.3%) isolates were classified as chloroquine resistant P. vivax isolates. Seven non-synonymous mutations and 2 synonymous were identified in Pvmdr1 gene. Y976F and F1076L mutations were detected in 7 (23.3%) and 16 isolates (53.3%), respectively. Analysis of Pvcrt-o gene revealed that all isolates were wild-type. Our results suggest that chloroquine resistance gene is now spreading in this area. Monitoring of chloroquine resistant molecular markers provide a useful tool for future control of P. vivax malaria.
Databáze:	OpenAIRE
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