| Autor: |
Alex Carisey, Ricky Tsang, Matthew J. Stroud, Christoph Ballestrem |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781617792069 |
| Popis: |
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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