| Autor: |
Joshua M. Lensmire, Elliot Ensink, Michael R. Wischer, Martha H. Mulks, Lo M. Sosinski, Neal D. Hammer, Jack P. Dodson, Sophia Y. Lunt, Daniel H. Havlichek, John C. Shook, Christopher C Cooper, Janani Ravi, Phillip C. Delekta |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.10.26.465763 |
| Popis: |
Sulfur is an indispensable element for proliferation of bacterial pathogens. Prior studies indicated that the human pathogen, Staphylococcus aureus utilizes glutathione (GSH) as a source of nutrient sulfur; however, mechanisms of GSH acquisition are not defined. Here, we identify a previously uncharacterized five-gene locus comprising a putative ABC-transporter and γ–glutamyl transpeptidase (ggt) that promotes S. aureus proliferation in medium supplemented with either reduced or oxidized GSH (GSSG) as the sole source of nutrient sulfur. Based on these phenotypes, we name this transporter the Glutathione import system (GisABCD). We confirm that Ggt is capable of cleaving GSH and GSSG γ–bonds and that this process is required for their use as nutrient sulfur sources. Additionally, we find that the enzyme is cell associated. Bioinformatic analyses reveal that only Staphylococcus species closely related to S. aureus encode GisABCD-Ggt homologues. Homologues are not detected in Staphylococcus epidermidis. Consequently, we establish that GisABCD-Ggt provides a competitive advantage for S. aureus over S. epidermidis in a GSH-dependent manner. Overall, this study describes the discovery of a nutrient sulfur acquisition system in S. aureus that targets GSH and promotes competition against other staphylococci commonly associated with the human microbiota. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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