Cis-9, trans-11-Conjugated Linoleic Acid Inhibits Allergic Sensitization and Airway Inflammation via a PPAR γ-Related Mechanism in Mice3
| Autor: | Paolo Maria Matricardi, A. Avagyan, M. Krokowski, Yasemin Darcan, Anke Jaudszus, Eckard Hamelmann, Peter Möckel, Gerhard Jahreis |
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| Rok vydání: | 2008 |
| Předmět: |
Allergy
Nutrition and Dietetics biology business.industry Conjugated linoleic acid food and beverages Medicine (miscellaneous) respiratory system Pharmacology Immunoglobulin E medicine.disease respiratory tract diseases Allergic sensitization chemistry.chemical_compound Ovalbumin medicine.anatomical_structure chemistry Eicosanoid Immunology biology.protein Medicine Arachidonic acid business Sensitization |
| Zdroj: | The Journal of Nutrition. 138:1336-1342 |
| ISSN: | 0022-3166 |
| Popis: | Milk consumption from early childhood on has been found to be inversely correlated with allergic sensitization and the onset of bronchial asthma. We tested whether cis-9,trans-11-conjugated linoleic acid (c9,t11-CLA), naturally occurring in milk fat, may prevent allergic sensitization and inhibit airway inflammation in a murine asthma model. BALB/c mice were fed a diet enriched in 1 wt% ofc9,t11-CLA or a control diet 7 d prior toand for 32 d during sensitization [d 1 and 14, 100 mg/L ovalbumin (OVA) in adjuvant vs. PBS] and airway challenges (d 28‐30, 1% OVA in PBS vs. PBS). Subgroups of mice were coadministered 20 mmol/L of the selective PPARg antagonist GW9662 during each OVA challenge. C9,t11-CLA feeding resulted in significantly reduced IgE production and allergen-induced in vivo airway hyperresponsiveness. Further, less mucous plugging of segmental bronchi and significantly reduced interleukin-5 and eosinophils were determined in bronchoalveolar lavage fluids of c9,t11-CLA-fed mice. C9,t11-CLA feeding prevented the downregulation of PPARg mRNA in the lung tissues observed after allergen sensitization and airway challenges in control mice. The inhibitory effects of c9,t11-CLA on airway inflammation were partially prevented by coadministration of GW9962. Further, c9,t11-CLA feeding resulted in a significantly lower concentration of the eicosanoid precursor, arachidonic acid, in tissue lipids. These findings demonstrate that dietary c9,t11-CLA can reduce allergic airway inflammation, most likely via a PPARg-related mechanism and by reducing eicosanoid precursors. They give new insights into the fatty acid-mediated mechanism of immunomodulation and may represent a step toward an attractive novel strategy in the dietary prevention and treatment of allergic asthma. J. Nutr. 138: 1336‐1342, 2008. |
| Databáze: | OpenAIRE |
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