| Popis: |
The properties of the colloidal SERS probes are crucial for the success of SERS microscopic experiments. Stability and robustness, sensitivity as well as steric accessibility for bioconjugation are few very important aspects. Figure 1 (left) shows a hydrophilic SERS probe stabilized by many short and few longer spacer units [3]. A second approach, based on the glass encapsulation for stabilizing SERS labels, is schematically depicted in Figure 1 (right). This type of SERS probes was optimized for red laser excitation in order to improve image contrast by minimizing unwanted autofluorescence of biological specimens [4]. Two different routes to the glass encapsulation of a complete monolayer of Raman reporters will be described [4,5]. |
