Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids
| Autor: | Stacy L. Capehart, Ioana L. Aanei, Matthew B. Francis, Jeff E. Glasgow |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
biology viruses Nanoparticle 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences 0104 chemical sciences Amino acid Bacteriophage chemistry.chemical_compound chemistry Chemical engineering Capsid Colloidal gold Surface charge Self-assembly 0210 nano-technology DNA |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493978069 |
| DOI: | 10.1007/978-1-4939-7808-3_21 |
| Popis: | Encapsulation into virus-like particles is an efficient way of loading cargo of interest for delivery applications. Here, we describe the encapsulation of proteins with tags comprising anionic amino acids or DNA and gold nanoparticles with negative surface charges inside MS2 bacteriophage capsids to obtain homogeneous nanoparticles with a diameter of 27 nm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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