An algorithm for identification of CTX-M-type beta-lactamase coding genes using restriction fragment length polymorphism analysis of the PCR-product
| Autor: | E. N. Kuzhelnaya, I. V. Abaev, S. D. Pryamchuk, V. A. Anisimova, Nadezhda K. Fursova, Edward A. Svetoch, Yu. N. Kovalev |
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| Rok vydání: | 2011 |
| Předmět: |
Genetics
medicine.medical_treatment Pcr cloning Biology biology.organism_classification Microbiology Terminal restriction fragment length polymorphism Infectious Diseases Virology GenBank Beta-lactamase medicine Identification (biology) Restriction fragment length polymorphism Molecular Biology Algorithm Gene Bacteria |
| Zdroj: | Molecular Genetics, Microbiology and Virology. 26:146-153 |
| ISSN: | 1934-841X 0891-4168 |
| Popis: | An algorithm for step-by-step identification of blaCTX-M genes determining the resistance to cephalosporins III–IV in gram-negative bacteria is developed. The algorithm allows identification of 49 genes out of 96, which are already present in the GenBank database. The remaining 47 genes are identified in several small groups of 2 to 6 genes, excluding the blaCTX-M-14-like subgroup, which comprises 13 genes. The blaCTX-M genes’ identification is carried out with two-step restriction fragment length polymorphism analysis (PCR-RFLP) of a 544-bp-long PCR-product. |
| Databáze: | OpenAIRE |
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