Extended shelf life of enzyme-based biosensors using a novel stabilization system
| Autor: | J.R. Woodward, S. Parker, I.J. Higgins, J.N. Hulbert, Tim Gibson |
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| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Biomedical Engineering Biophysics Enzyme electrode General Medicine Horseradish peroxidase Amperometry Alcohol oxidase chemistry.chemical_compound chemistry Electrochemistry biology.protein Sugar alcohol Hydrogen peroxide L-glutamate oxidase Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 7:701-708 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/0956-5663(92)85052-c |
| Popis: | The storage stability of amperometric enzyme electrodes has been enhanced by a combination of a soluble, positively charged polymer, diethylaminoethyl (DEAE)-dextran, and a sugar alcohol, lactitol. Two different types of alcohol biosensor have been produced using the enzyme alcohol oxidase, isolated from the methylotrophic yeast Hansenula polymorpha. The first employs enzyme entrapment between two membranes with direct hydrogen peroxide amperometry at +0·65 V. The second was based on the mediated, coupled reaction with horseradish peroxidase and N-methyl phenazimiumtetracyanoquinonedimethane (NMP-TCNQ) on a graphite electrode. In both cases, addition of the stabilizers promoted a considerable increase in the storage stability of the enzyme component, as indicated by an increase in the shelf life of desiccated biosensors under conditions of thermal stress at 37°C. In addition, an L-glutamate biosensor constructed from NMP-TCNQ-modified graphite electrodes and L-glutamate oxidase also exhibited an increase in shelf life when stored, desiccated in the presence of stabilizers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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