263 Fecal Microbial Transplantation Can Modulate Increased Colitis Susceptibility Induced by High Sugar Diets
| Autor: | Karen Madsen, Ruth Ginter, R Fedorak, Ammar Hassanzadeh Keshteli, Ambika Agrawal, Naomi Hotte |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
endocrine system diseases Hepatology biology business.industry digestive oral and skin physiology Gastroenterology medicine.disease biology.organism_classification digestive system Ulcerative colitis digestive system diseases Liver disorder Primary sclerosing cholangitis Transplantation Fusobacterium Internal medicine medicine Colitis Roseburia business Dysbiosis |
| Zdroj: | Gastroenterology. 148:S-60 |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/s0016-5085(15)30208-0 |
| Popis: | Background: Primary sclerosing cholangitis (PSC) is a cholestatic liver disorder very frequently associated with inflammatory bowel diseases (IBD). The intestinal microbiota seems to be essential in PSC as bacterial translocation is thought to play an important role in the pathogenesis of the disease. Given the association with IBD and the fact that patients with IBD have a well-documented intestinal dysbiosis, we hypothesized that dysbiosis would also play a role in PSC. Methods: Faecal samples from 52 PSC patients and 52 age, sex and BMI matched healthy controls were collected. Within the PSC cohort, 39 patients had concomitant IBD (17 Crohn's disease (CD) and 22 ulcerative colitis (UC)). After bacterial DNA extraction, 16S rDNA paired-end sequencing was performed using Illumina MiSeq sequencer. Successfully combined reads were quality-filtered (30 QS over 90% of read length) and chimeric sequences filtered out (UCHIME). Sequencing depth was downsized to 10000 reads/sample by random selection. Reads were clustered at 97% sequence similarity for species-level de novo OTU picking (USEARCH). The Ribosomal Database Project classifier was used for taxonomic assignment. Statistical analyses were performed with R package phyloseq, using non-parametric Mann-Whitney U and Kruskal-Wallis tests, with multiple testing correction (FDR). Results: The overall microbiota diversity was significantly decreased in PSC patients compared to healthy controls (p |
| Databáze: | OpenAIRE |
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