Identification of MAVS as a Novel Risk Factor for the Development of Osteoarthritis
| Autor: | Lingyun Tang, En-Ning Zhang, Jie Liu, Zhugang Wang, Hong-Xin Zhang, Shunyuan Lu, Yan-gui Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cartilage homeostasis RIG-I Signal transducing adaptor protein Single-nucleotide polymorphism Cell Biology Biology Pathology and Forensic Medicine Pathogenesis 03 medical and health sciences 030104 developmental biology Apoptosis Immunology Neurology (clinical) Viability assay Geriatrics and Gerontology Receptor |
| Zdroj: | Aging and disease. 9:40 |
| ISSN: | 2152-5250 |
| DOI: | 10.14336/ad.2017.0308 |
| Popis: | Evidence indicated that inflammatory response and some pattern-recognition receptors play important roles in the occurrence and progression of osteoarthritis. This study is conducted to evaluate the role of RIG-I and its adaptor protein MAVS in the pathogenesis of osteoarthritis. Four SNPs in RIG-I gene and four in MAVS gene were genotyped in 1056 Chinese Han population. We also overexpressed MAVS in murine chondrogenic ATDC5 cells and analyzed the cell viability and apoptosis. Rs11795343 (P-allele: 0.063394) in RIG-I, rs17857295 (P-allele: 0.073518) and rs7262903 (P-allele: 0.054052, P-genotype: 0.067930) in MAVS were marginally associated with OA. Rs7269320 (P-allele: 0.014783, P-genotype: 0.03272) in MAVS was significant associated with OA. Further analyses in different genders indicated that rs7262903 (P-allele: 0.017256, P-genotype: 0.045683) and rs7269320 (P-allele: 0.013073, P-genotype: 0.038881) are significantly associated with OA in female group. Haplotype analyses indicated G-C-G (χ2: 4.328, P-value: 0.037503) in rs10813821-rs11795343-rs659527 block of RIG-I, G-C-A-T (χ2: 4.056, P-value: 0.044028) and G-C-C-C (χ2: 14.295, P-value: 0.000158) in rs17857295-rs2326369-rs7262903-rs7269320 block of MAVS were significantly associated with OA. Furthermore, forced expression of MAVS could suppress the viability and promote the apoptosis of ATDC5 chondrogenic cells. In conclusion, this study indicated that RIG-I and MAVS are probably associated with OA in the females of Chinese Han population. And MAVS might be a novel risk factor for OA which may involve in growth of chondrocytes and cartilage homeostasis. |
| Databáze: | OpenAIRE |
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