Determination of Oxidation-Reduction Potentials of Sterile Culture Mediums with the Graphite Electrode
| Autor: | I. Forest Huddleson, C. D. Tuttle |
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| Rok vydání: | 1934 |
| Předmět: | |
| Zdroj: | Journal of Infectious Diseases. 54:273-279 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/54.2.273 |
| Popis: | It has already been demonstrated that the potentials of inorganic chemical oxidation-reduction systems and of a few organic ones can be measured without difficulty at the surface of a bright noble metal electrode with the conventional potentiometer. On the other hand, in many organic oxidation-reduction systems, it is difficult to make measurements with bright noble metal electrodes by the use of the conventional potentiometer owing to the ease with which the electrodes are polarized. It has been shown that this difficulty is increased in organic systems that are poorly poised.1 Bacteriologic culture mediums are examples of extremely poorly poised organic systems. Four general methods have been employed to overcome or to avoid polarization and its effects. They are: the use of oxidation-reduction dyes to indicate the extent of oxidation or reduction through their color changes,2 the use of a vacuum tube voltmeter, of a potentiometer with a vacuum tube arrangement or of an electrometer as the null instrument,3 the use of poising effects, such as those produced by poising substances, the passage of highly purified nitrogen or the exclusion of air4 and the use of an electrode that is not easily polarized and that permits of the use of the conventional potentiometer.5 In the light of previous studies of this nature, it appears desirable to find an electrode which will materially reduce the laborious technic |
| Databáze: | OpenAIRE |
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