1842-P: Enhanced TGF-ß1 Signaling in the Ducts of Cystic Fibrosis Pancreas Increases Basal Insulin Secretion by Islets
| Autor: | Andrew W. Norris, Yunxia O'Malley, Idil Apak-Evans, Aliye Uc, Pavana G. Rotti, John F. Engelhardt |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Forskolin Carbachol IBMX biology Chemistry Endocrinology Diabetes and Metabolism medicine.disease Cystic fibrosis Cystic fibrosis transmembrane conductance regulator chemistry.chemical_compound medicine.anatomical_structure Endocrinology Secretory protein Internal medicine Internal Medicine medicine biology.protein Secretion Pancreas medicine.drug |
| Zdroj: | Diabetes. 68 |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | Cystic fibrosis (CF) is a multi-organ disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CFTR protein is most highly expressed in pancreatic ductal epithelium, where loss of its function leads to ductal plugging, acinar destruction, and eventual diabetes. We hypothesize that exocrine pancreas remodeling in CF alters ductal paracrine signals to the endocrine pancreas. To test this hypothesis, we established primary ductal epithelium grown at an air-liquid interface (ALI) from WT and CFTR-KO ferret pancreas. Carbachol or IBMX/Forskolin (I/F) stimulated basolateral and apical secretions were analyzed for differentially secreted proteins using quantitative mass spectroscopy. Upon carbachol secretion 39 apical and 15 basolateral proteins were differentially secreted (P Disclosure P. Rotti: None. I. Apak-Evans: None. Y. OMalley: None. A. Norris: None. A. Uc: None. J. Engelhardt: None. Funding National Institutes of Health |
| Databáze: | OpenAIRE |
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