Phosphorescence quenching as an approach for estimating the localization of triplet label in cotton fibers

Autor: G. I. Likhtenshtein, I. Kh. Yusupov
Rok vydání: 2012
Předmět:
Zdroj: Biophysics. 57:197-200
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s000635091202025x
Popis: The method based on quantitative analysis of chromophore fluorescence (phosphorescence) quenching, for instance, by a stable nitroxide radical, was the first time used to measure the depth of immersion of a triplet label in cotton fiber as a molecular object. Erythrosine triplet labels were incorporated in cotton fibers with subsequent measurement of the efficiency of label phosphorescence quenching and of the temperature dependence of phosphorescence duration. Using the concept of dynamic quenching in solution and the empirical dependence of the parameters of static quenching between centers with fixed distance, we could estimate the depth of chromophore immersion in the fiber (7.4–7.8 dynamics of the label in the millisecond range of correlation times. Subtle differences in microstructure and molecular dynamics were revealed between fiber specimens from healthy and diseased cotton. The proposed approach can be used for investigating a wide scope of biological and nonbiological objects.
Databáze: OpenAIRE
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