Reverse transcription PCR-based detection of matrix and hemagglutinin-neuraminidase genes among Avian orthoavulavirus 1 clinical isolates in the Philippines, 1991-2017
| Autor: | Dennis V. Umali, Jorge Gil C. Angeles, Remil L. Galay, Jarel Elgin M. Tolentino |
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| Rok vydání: | 2021 |
| Předmět: |
Renewable Energy
Sustainability and the Environment viruses General Chemical Engineering General Chemistry Matrix (biology) Biology Biochemistry Molecular biology Reverse transcription polymerase chain reaction General Earth and Planetary Sciences General Agricultural and Biological Sciences Gene Hemagglutinin-neuraminidase General Environmental Science |
| Zdroj: | Research Journal of Chemistry and Environment. 25:89-95 |
| ISSN: | 2278-4527 0972-0626 |
| DOI: | 10.25303/259rjce8995 |
| Popis: | Newcastle disease (ND) is a highly infectious disease that affects devastatingly the avian population worldwide. It is caused by Avian orthoavulavirus 1 (AOAV-1), or better known as Newcastle disease virus belonging to phylum Negarnaviricota, class Monjiviricetes, order Mononegavirales and family Paramyxoviridae. This virus consists of six principal structural proteins namely: the nucleocapsid protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase protein (HN) and the large protein RNA dependent RNA polymerase (L).. The present study aimed to molecularly detect the M and HN gene segments of the AOAV-1 field isolates from clinical cases in the Philippines from 1991 through 2017. RT-PCR amplification and sequence analyses using primers NDV-For4359 and NDV-Rev4788 which anneal to the matrix gene and primers NDV-For6369 and NDV-Rev6598 targeting the HN genes, identified all isolates to be AOAV-1. Determining the different genes of the virus would greatly help scientists and researchers to accurately identify the viral isolates in order to improve epidemiological studies and surveillance of the disease in the country. |
| Databáze: | OpenAIRE |
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