Induction of collagenase secretion in human fibroblast cultures by growth promoting factors
| Autor: | Deborah E. Geiman, R. L. Ladda, Chu Chang Chua, George H. Keller |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Fibroblast growth factor receptor 2
Growth factor medicine.medical_treatment Cell Biology Fibroblast growth factor receptor 4 Fibroblast growth factor receptor 3 Biology Biochemistry Molecular biology Fibroblast growth factor receptor Epidermal growth factor Cell surface receptor Collagenase medicine Molecular Biology medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 260:5213-5216 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(18)89004-4 |
| Popis: | In human foreskin fibroblast cultures, two proteins with Mr 60,000 and 55,000 were found to be induced about 3.5-fold by epidermal growth factor (EGF), platelet-derived growth factor, and beta-transforming growth factor. The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. Collagenase enzyme activity in the medium from EGF-treated cultures was also induced at least 3-fold compared to control cultures. Induction of collagenase was dependent upon de novo protein and RNA synthesis and was observed in the medium 10 h after addition of EGF. Although these growth-promoting factors interact with separate membrane receptors, each induced the secretion of a common protein, suggesting that collagenase may be important in some aspect of mitogenesis, cell mobilization, and migration. |
| Databáze: | OpenAIRE |
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