Dose-response and Kinetics of the Formation of DNA Adducts in the EarthwormEisenia Fetida AndreiExposed to B(A)P-contaminated Artificial Soil
| Autor: | D. Ribera, M Saint-Denis, J. F. Narbonne, A. Pfohl-leszkowicz |
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| Rok vydání: | 2000 |
| Předmět: |
Eisenia fetida
Polymers and Plastics biology Organic Chemistry Earthworm biology.organism_classification medicine.disease_cause Soil contamination chemistry.chemical_compound chemistry Benzo(a)pyrene Environmental chemistry Materials Chemistry medicine Bioassay Pyrene Steady state (chemistry) Genotoxicity |
| Zdroj: | Polycyclic Aromatic Compounds. 18:117-127 |
| ISSN: | 1563-5333 1040-6638 |
| DOI: | 10.1080/10406630008028140 |
| Popis: | Earthworms, Eisenia fetida andrei (E. f. a.), were exposed to soils contaminated with increasing concentrations of benzo(a)pyrene for different periods of time to investigate: the possible metabolism pathways of B(a)P; the toxicological significance of DNA adduct formation in the worm; and the suitability of the 32P-postlabelling method as a bioassay for chemical toxicity testing and for surveys of soil contamination. Our results indicate that B(a)P could be metabolized via either cytochrome P-450 dependent activities or free radical oxidation. For short-term exposures, the level of DNA adducts formed was dose-dependent and could thus be considered toxicologically significant. At the highest doses, the formation of DNA adducts reached a steady state. Moreover, the 32P-postlabelling method is particularly sensitive, as DNA adducts was significant upon exposure to just 50 μg B(a)P/kg artificial soil. In conclusion, the method used and the species, E. f. a., are potential tools in chemicals or conta... |
| Databáze: | OpenAIRE |
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