TLC detection of chemical interactions of vitamins A and D with drugs

Autor: Marica Medić-Šarić, Alan Ramić, Ivona Jasprica, Srećko Turina
Rok vydání: 2006
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 19:27-31
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.19.2006.1.5
Popis: Summary Thin-layer chromatography has been used to investigate possible chemical interactions of vitamins A and D with frequently used therapeutics (estrogens and progestins, corticosteroids, HMG CoA reductase inhibitors, vitamins, and nonsteroidal anti-inflammatory drugs). We also compared our results with biochemical interactions found in the literature. Concentrations of vitamins and drugs applied to the plate were adjusted to mimic the doses usually prescribed in therapy. TLC was performed on 10 cm × 20 cm TLC plates precoated with silica gel 60 F254. The first step was to determine R F values for each vitamin and drug using three different mobile phases – cyclohexane–ether, 50 + 50 (v/v), cyclohexane–ether, 85 + 15 (v/v), and ethyl acetate. Solutions of vitamins A and D were applied to the plates as 8 mm bands and the investigated drugs were applied as spots. Chromatography was performed in ascending mode. After calculation of RF values we used a combination of stationary and mobile phase for which overlapping of the vitamin band and drug spot occurred during chromatogram development. The strength of interaction was measured as a surface below or above the distorted part of the sample band, visible under short wavelength UV light (λ = 254 nm) or after exposing the plates to iodine vapor. The chromatograms were documented by use of a Camag Reprostar 3 System. We established the occurrence of chemical interactions between vitamin A and estradiol propionate, tocopherol acetate, ibuprofen, and ketoprofen, and between vitamin D and hydrocortisone, medroxyprogesterone acetate, norethisterone acetate, aspirin, ketoprofen, and acetaminophen. A clear relationship between chemical and biochemical interactions could not be established.
Databáze: OpenAIRE
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