Abstract 2596: Spliced messenger RNA of tumor-educated platelets yields a new diagnostic prospective for pancreatic cancer
| Autor: | Heleen Verschueren, Myron G. Best, Thomas Wurdinger, Maarten F. Bijlsma, Elisa Giovannetti, Laura L. Meijer, Ewoud van der Lelij, Marc G. Besselink, Niccola Funel, Geert Kazemier, Giulia Mantini, Ingrid Garajová, Nik Sol, François Rustenburg, Sjors G. J. G. In ‘t Veld, Tessa Y S Le Large |
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| Rok vydání: | 2018 |
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| Zdroj: | Cancer Research. 78:2596-2596 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed at advanced disease stages and invasive procedures are needed to confirm diagnosis. Innovative liquid biopsy-based approaches are warranted to diagnose early stages and prevent unnecessary treatment for advanced disease or misdiagnosed benign diseases. CA19.9 is the only clinically implemented biomarker, but its sensitivity is inadequate and it lacks specificity. Spliced messenger RNA (mRNA) of tumor-educated platelets (TEPs) emerged recently as new potent pan-cancer biomarker (Best et al, Cancer Cell 2015&2017). The aim of this study was to evaluate PDAC-specific TEP profiles as diagnostic and staging biomarker. Samples were collected at four international academic centers. Firstly, TEPs were isolated from blood of 135 PDAC patients including early disease (stage I-IIb), advanced disease (stage III) and metastatic patients (stage IV). In addition, 250 age-matched healthy controls (HC) and 72 patients with a benign disease in the pancreaticobiliary tract were included to create a heterogeneous control group that reflects the real patient population at the outpatient clinic. After total RNA was isolated, SMARTer amplification was performed and whole transcriptome mRNA was sequenced on the Illumina platform. Intron-spanning platelet mRNA reads were selected for further differential expression analysis. PDAC patients had a significant different mRNA TEP profile compared to HC (p-value Citation Format: Tessa Ya Sung Le Large, Laura L. Meijer, Sjors G. In ‘t Veld, Nik Sol, Myron G. Best, Giulia Mantini, Ewoud van der Lelij, Francois Rustenburg, Heleen Verschueren, Niccola Funel, Ingrid Garajova, Maarten F. Bijlsma, Marc G. Besselink, Elisa Giovannetti, Tom Wurdinger, Geert Kazemier. Spliced messenger RNA of tumor-educated platelets yields a new diagnostic prospective for pancreatic cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2596. |
| Databáze: | OpenAIRE |
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