PHARMACOLOGICAL REGULATION OF DIGESTION IN THE ANAUTOGENOUS FLESH FLY,Sarcophaga crassipalpis, BY SIMPLE INJECTION OF 6-HYDROXYDOPAMINE
| Autor: | Roger Huybrechts, Magdalena Bil |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology medicine.medical_treatment Sarcophaga Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Hydroxydopamine Protease biology Flesh fly fungi Midgut General Medicine biology.organism_classification Trypsin 030104 developmental biology Endocrinology Insect Science Vitellogenesis Sarcophaga crassipalpis 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 91:137-151 |
| ISSN: | 0739-4462 |
| Popis: | Female anautogenous Sarcophaga flesh flies need a protein meal to start large-scale yolk polypeptides (YPs) production and oocyte maturation. Protein meal rapidly elicits a brain-dependent increase in midgut proteolytic activity. Trypsin and chymotrypsin together represent over 80% of protease activity in liver-fed flies. Abdominal injection of 6-hydroxydopamine (6-OHDA) dose-dependently prohibits this increase in proteolytic activity at translational level in a similar way as post liver feeding decapitation. Delayed injection of 6-OHDA later than 6 h post liver meal has no effect. In flesh flies, chemical decapitation by 6-OHDA, by interrupting the brain-gut dopaminergic signaling, can be used as tool for the controlled inhibition of midgut proteolytic activity and subsequent ovarial development. Inhibition of ovarial development is probably indirect due to a deficit in circulating amino acids needed for YPs synthesis. |
| Databáze: | OpenAIRE |
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