Influence of temperature and soil water content on bacterial, archaeal and denitrifying microbial communities in drained fen grassland soil microcosms

Autor: Simona Leskovec, Blaž Stres, Tjaša Danevčič, Janez Hacin, Ivan Mahne, Lara Resman, Ines Mandic-Mulec, Mirna Mrkonjić Fuka, Levin Pal, David Stopar
Rok vydání: 2008
Předmět:
Zdroj: FEMS Microbiology Ecology. 66:110-122
ISSN: 1574-6941
0168-6496
DOI: 10.1111/j.1574-6941.2008.00555.x
Popis: In this study, microcosms were used to investigate the influence of temperature (4 and 28 °C) and water content (45% and 90% WHC) on microbial communities and activities in carbon-rich fen soil. Bacterial, archaeal and denitrifier community composition was assessed during incubation of microcosms for 12 weeks using terminal restriction fragment length polymorphism (T-RFLP) profiling of 16S rRNA and nitrous oxide reductase ( nosZ ) genes. In addition, microbial and denitrifier abundance, potential denitrification activity and production of greenhouse gases were measured. No detectable changes were observed in prokaryote or denitrifier abundance. In general, cumulatively after 12 weeks more carbon was respired at the higher temperature (3.7 mg CO2 g−1 soil), irrespective of the water content, whereas nitrous oxide production was greater under wet conditions (98–336 μg N2O g−1 soil). After an initial lag phase, methane emissions (963 μg CH4 g−1 soil) were observed only under warm and wet conditions. T-RFLP analyses of bacterial 16S rRNA and nosZ genes revealed small or undetectable community changes in response to temperature and water content, suggesting that bacterial and denitrifying microbial communities are stable and do not respond significantly to seasonal changes in soil conditions. In contrast, archaeal microbial community structure was more dynamic and was strongly influenced by temperature.
Databáze: OpenAIRE
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