Major Genetic Determinants of Extended-Spectrum Β-Lactamase (ESBL), Carbapenemase, Fosfomycin and Colistin Resistance in Escherichia Coli from Intensive Care Units
| Autor: | Ahmed Mhawesh, Marwa khudair, Omer N. Abbas |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Bionatura. 7:1-5 |
| ISSN: | 1390-9355 1390-9347 |
| DOI: | 10.21931/rb/2022.07.01.11 |
| Popis: | Escherichia coli (E. coli) strains placed in predominant nosocomial bacteria in intensive care units (ICUs), resulting in severe drug-resistant infections. Non-susceptibility to β-lactams and last-line drugs such as Fosfomycin and Colistin cause limited availability of infections eradication. The objective of this study included the determination of genes encoding extended-spectrum β-lactamase (ESBL), Carbapenemase, Colistin, and Fosfomycin resistance in clinical isolates of E. coli in ICUs. A total of 200 E. coli isolates were identified from ICU settings. The CTXM-1, SHV, IMP and OXA-48 genes were detected for β-lactamases using the polymerase chain reaction (PCR) technique. The fosA3 and mcr-1 and mcr-2 genes were also detected for resistance against Fosfomycin and Colistin. The CTX-M1, SHV, IMP and OXA-48 genes were detected in 60 (30%), 56 (28%), 28 (14%) and 4 (8%) of isolates. none of the E. coli isolates had the mcr-2 and fosA3 genes. Despite the existence of resistance genes to the third-generation antibiotics and Carbapenemase s, any isolates had genes for resistance to Fosfomycin and Colistin. More studies are needed to follow the resistance genes against last-resort antibiotics. |
| Databáze: | OpenAIRE |
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