Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach
| Autor: | Hay-Yan J Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Materials science 010401 analytical chemistry Tissue Processing Rat brain Mass spectrometry 01 natural sciences digestive system diseases Mass spectrometry imaging 0104 chemical sciences Matrix (chemical analysis) 03 medical and health sciences Matrix-assisted laser desorption/ionization Ischemic stroke Brain section 030304 developmental biology Biomedical engineering |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614099 |
| DOI: | 10.1007/978-1-0716-1410-5_20 |
| Popis: | Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) of lipids is considered one of the shotgun lipidomic techniques that explores the in situ distribution of lipids in tissue sections. To successfully perform this task, knowledge and experience in the conventional cryosection, tissue section collection and handling, and mass spectrometry data analysis and signal processing are needed. A MALDI-MSI protocol covering from the fresh organ collection, cryosection and tissue processing, matrix application, MSI data acquisition, to the final MSI display of lipid distribution in the brain section of an ischemic stroke rat is described to exemplify this technique. Due to the multidisciplinary nature of this approach, plenty of preparation, practice, and familiarization to several critical steps ahead of the engagement with actual biological samples are needed to ensure a successful MALDI-MSI presentation of lipids in situ. |
| Databáze: | OpenAIRE |
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