Cloning, sequencing of the HSC70 gene in Ctenopharyngodon idella

Autor: Hong-ju Chen, Chun-Ge Li, Yong Fu, Xiangshan Ji, Feng Liu, Zhongdian Dong
Rok vydání: 2014
Předmět:
Zdroj: Wuhan University Journal of Natural Sciences. 19:235-244
ISSN: 1993-4998
1007-1202
Popis: A cDNA encoding heat shock cognate protein 70 (HSC70) was cloned from liver of grass carp (Ctenopharyngodon idella) (GenBank JF436930). This cDNA was found out to contain 2 346 bp in length, including 1 950 bp of complete coding sequence encoding 649 amino acids (aa), plus 89 bp of 5′-UTR and 307 bp of 3′-UTR. Analysis of its genomic structure revealed that its corresponding gene contained seven exons and six introns. Homology analysis indicated that it shared 99% of identity with HSC70 of breams and 86% of identity with HSP70 of Drosophila. Fluorescent RT-PCR analysis revealed that at 28 °C, this gene was expressed in abdominal fat, muscle, intestines, brain, middle kidney, head kidney, gonads, swim bladder, liver, heart, spleen, gills, and fins with expression level in liver being the highest (p
Databáze: OpenAIRE
Externí odkaz: