Tanshinone IIA Suppresses Non-Small Cell Lung Cancer Through Beclin-1-Mediated Autophagic Apoptosis
| Autor: | Elaine Lai-Han Leung, Lei Yang, Shasha Bai, Jing Bai, Wenhao Wen, Yuan Zheng, Rong Zhang, Sainan Cui, Zhongqiu Liu, Yongfei Cui, Huiyuan Lin |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Caspase-9
education.field_of_study Environmental Engineering integumentary system General Computer Science biology Chemistry Materials Science (miscellaneous) General Chemical Engineering ATG5 Autophagy General Engineering Energy Engineering and Power Technology Caspase 3 BECN1 Salvia miltiorrhiza Sequestosome 1 Apoptosis biology.protein Cancer research education |
| Zdroj: | Engineering. 19:128-138 |
| ISSN: | 2095-8099 |
| Popis: | It is necessary to develop a new strategy for treatment of lung cancer since it is the main cause of cancer death. Tanshinone IIA (Tan IIA), an active ingredient of a commonly used traditional Chinese herb Salvia miltiorrhiza, provides a new direction to develop a new strategy for the treatment. It has been found that Tan IIA could inhibit lung cancer in vitro and in vivo by inducing autophagic apoptosis. Tan IIA increased apoptotic cells and the expression of cleaved caspase 3 and cleaved caspase 9, decreased B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) ratio in human non-small cell lung cancer (NSCLC) cell lines, which was promoted by an autophagy activator Rapamycin, and weaken by autophagy inhibitor 3-methyladenine (3-MA). In addition, Tan IIA induced more autophagosomes, up-regulated light chain 3β (LC-3B) I and LC-3B II conversion and less sequestosome 1 (SQSTM1/p62) expression, which cannot be weakened by the caspase 3 antagonist. Moreover, overexpression of LC-3B gene (LC3B) and downregulation of autophagic gene 5 (ATG5) further confirmed that Tan IIA induced autophagic apoptosis in NSCLC cell lines. Beclin-1 gene (BECN1) overexpression and silence attenuated the effects of Tan IIA, suggesting autophagic apoptosis that Tan IIA induced was dependent on Beclin-1. Overall, our study demonstrated a new treatment mechanism of Tan IIA and suggested that Tan IIA is a potential new anti-cancer therapeutic option. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|