| Autor: |
Andreeva N.S., Dergousova N.I., Leonova Yu F., Zinchenko A.A.I, Rumsh L.D. |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Protein & Peptide Letters. 4:321-328 |
| ISSN: |
0929-8665 |
| DOI: |
10.2174/092986650405221017152834 |
| Popis: |
Abstract: An extensive net of hydrogen bonds including the active carboxyls and Tyr residue locating in the so-called 'flap' in pepsin-like enzymes was planned to be restored in molecule of HIV-1 protease. For this purpose, 5 mutations and 2 deletions were introduced simultaneously in the enzyme. Some of the residues subjected to mutagenesis are known to be changed during formation of drug resistant HIV-1 protease. The obtained mutant was fully active, but had an altered specificity and pH optimum. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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