Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans
| Autor: | Luana Marotta Reis de Vasconcellos, Luciane Dias de Oliveira, Samira Esteves Afonso Camargo, Gabriela de Fátima Santana-Melo, Jonatas Rafael de Oliveira |
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| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry Biofilm 02 engineering and technology 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Streptococcus mutans Corpus albicans Rosmarinus Lowry protein assay Officinalis 0202 electrical engineering electronic engineering information engineering 020201 artificial intelligence & image processing Food science 0210 nano-technology Candida albicans General Dentistry |
| Zdroj: | Brazilian Dental Science. 22:260-266 |
| ISSN: | 2178-6011 |
| DOI: | 10.14295/bds.2019.v22i2.1679 |
| Popis: | Objective: The resistance of fungi and bacteria to the available antimicrobials has increased and the development of alternative products to control them has become a very requirement. The use of plant products could be a viable option due to the efficacy, viability, and availability they present. Thereby, this study evaluated the effect of R. officinalis L. extract on C. albicans and S. mutans biofilms, by the total protein level analysis presented by the microorganisms. Material and Methods: For this purpose, monomicrobial biofilms were formed for 48 h and exposed to the R. officinalis L. extract for 5 min. Then, total protein quantification was performed by Lowry method. Results: The analysis showed significant total protein reduction of the biofilms after exposure to the plant extract, with 39 ± 11%, for C. albicans , and 32 ± 11%, for S. mutans. Conclusion: R. officinalis L. extract decreased the total protein level in both biofilms. Thus, C. albicans and S. mutans protein composition could be a target for action of antimicrobial agents. Keywords Biofilms; Candida albicans ; Proteins; Rosmarinus officinalis; Streptococcus mutans. |
| Databáze: | OpenAIRE |
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