MOF-5(Zn)-Fe 2 O 4 nanocomposite based magnetic solid-phase microextraction followed by HPLC-UV for efficient enrichment of colchicine in root of colchicium extracts and plasma samples
| Autor: | Mohammad Javad Khoshnood Mansoorkhani, Sonia Bahrani, Abbas Ostovan, Kheibar Dashtian, Mehrorang Ghaedi, Amin Salehi |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Sorbent Chromatography Chemistry Elution Sonication 010401 analytical chemistry Clinical Biochemistry 02 engineering and technology Cell Biology General Medicine 021001 nanoscience & nanotechnology Solid-phase microextraction 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Solvent chemistry.chemical_compound Desorption 0210 nano-technology Ethylene glycol |
| Zdroj: | Journal of Chromatography B. 1067:45-52 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.09.044 |
| Popis: | In present work, facile method is developed for determination of colchicine in human plasma sample, autumn and spring root of colchicium extracts by ultrasound assisted dispersive magnetic solid phase microextraction followed by HPLC-UV method (UAD-MSPME-HPLC-UV). Magnetic (Fe2O4-nanoparticles) metal organic framework-5, (MOF-5(Zn)-Fe2O4NPs) was synthesized by dispersing MOF-5 and Fe(NO3)3.9H2O in ethylene glycol (as capping agent) and NaOH (pH adjustment agent) by hydrothermal method. The prepared sorbent was characterized via XRD and SEM analysis and applied as magnetic solid phase in UAD-MSPME-HPLC-UV method. In this method, colchicine molecules were sorbed on MOF-5(Zn)-Fe2O4NPs sorbent by various mechanisms like ion exchange, hydrogen bonding and electrostatic, ᴨ-ᴨ, hard-hard and dipole-ion interaction followed by exposing sonication waves as incremental mass transfer agent and then the sorbent was separated from the sample matrix by an external magnetic fields. Subsequently, accumulated colchicine were eluted by small volume of desorption organic solvent. Influence of operational variables such as MOF-5(Zn)-Fe2O4NPs mass, volume of extracting solvent and sonication time on response property (recovery) were studied and optimized by central composite design (CCD) combined with desirability function (DF) approach. Under optimum condition, the method has wide linear calibration rang (0.5-1700ngmL-1) with reasonable detection limit (0.13ngmL-1) and R2=0.9971. Finally, the UAD-MSPME-HPLC-UV method was successfully applied for determination of colchicine autumn and spring root of colchicium extracts and plasma samples. |
| Databáze: | OpenAIRE |
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