Genetics and human conception: DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease
| Autor: | G. Levinson, David Bick, Joy C. Wu, Joseph D. Schulman, K Keyvanfar, E.F. Fugger, Lisa Dennison-Lagos, Rita A. Fields, Lucrecia Calvo, Karin M. Starr, Michael E. Sisson, Susan H. Black, Frances T. Palmer, Gary Harton, Richard J. Sherins |
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| Rok vydání: | 1995 |
| Předmět: |
Fetus
medicine.diagnostic_test Rehabilitation Obstetrics and Gynecology Embryo Biology Sperm Embryo transfer law.invention Andrology medicine.anatomical_structure Reproductive Medicine law embryonic structures Biopsy Multiplex polymerase chain reaction Immunology medicine Blastocyst reproductive and urinary physiology Polymerase chain reaction |
| Zdroj: | Human Reproduction. 10:979-982 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a136081 |
| Popis: | We report the world's first clinical pregnancy resulting from DNA-based enrichment for X-bearing human spermatozoa, for prevention of X-linked hydrocephalus. Sperm separation was followed by embryo biopsy and nested multiplex polymerase chain reaction (PCR) for gender determination. Enriched populations of X- bearing spermatozoa ranging from 80 to 89% pure as determined by fluorescence in-situ hybridization (FISH) resulted in in-vitro fertilization (IVF) rates indistinguishable from normal IVF procedures (65%). In two separate biopsy procedures, 7/9 and 15/16 of the resulting embryos were determined to be female by multiplex PCR. Embryo transfer resulted in a karyotypically normal female fetus. This technique should be widely applicable to gender selection for the prevention of genetic disorders |
| Databáze: | OpenAIRE |
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