A rapid and efficient method for the isolation of mitochondrial DNA from angiosperm tree species. Application to the restriction fragment length polymorphism distinction between European and American ashes

Autor:	Christian Raquin, M. E. Morand, Nathalie Frascaria-Lacoste, F. Vedel
Rok vydání:	2001
Předmět:	Mitochondrial DNA Ecology Organic solvent food and beverages Biology Isolation (microbiology) medicine.disease_cause Biochemistry General Biochemistry Genetics and Molecular Biology Gravity flow Pollen Botany medicine Microsatellite Restriction fragment length polymorphism Tree species
Zdroj:	Molecular Ecology Notes. 1:67-70
ISSN:	1471-8278
DOI:	10.1046/j.1471-8278.2001.00028.x
Popis:	A simplified method for the isolation of mitochondrial DNA (mtDNA) from several angiosperm tree species is described. The procedure does not require gradient ultracentrifugation or organic solvent extractions and can be performed in a single day. mtDNA was isolated from flower buds collected from adult trees just after the first pollen mitosis and purified by gravity flow through disposable columns containing an anion-exchange resin. The method led to restrictable mtDNA from the tree species analysed. Restriction patterns enabled us to distinguish between European and American ashes.
Databáze:	OpenAIRE
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