Association between histone hyperacetylation status in memory T lymphocytes and allergen-induced eosinophilic airway inflammation
| Autor: | Zeng Li Wang, Juan-Juan Fu, Gang Wang, Tao Fan, Hong Ping Zhang, Lei Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine biology HDAC11 business.industry medicine.medical_treatment Interleukin Inflammation Histone acetyltransferase 03 medical and health sciences 030104 developmental biology Histone Cytokine Trichostatin A Immunology biology.protein medicine Histone deacetylase medicine.symptom business medicine.drug |
| Zdroj: | Respirology. 21:850-857 |
| ISSN: | 1323-7799 |
| Popis: | Background and objective T lymphocytes, which are characterized by longevity and immune memory, play an important role in airway inflammation in asthma. Here, we assessed the association between immune memory and histone deacetylation and/or acetylation status. Methods CD4 + CD45RBlow cells (memory T (Tm)) obtained from the spleens of asthma mice models were co-cultured with glucocorticoids (GCs), trichostatin A (TSA) or anacardic acid (AA) and adoptively transferred to naive mice. Interleukin (IL)-4, 5 and 13 and IFN-γ concentrations were measured in culture supernatants and bronchoalveolar lavage fluid (BALF). Histone deacetylase (HDAC) and histone acetyltransferase (HAT) activities and the expression of T-bet, GATA-3, HDACs 1–11 and alveolar eosinophilic inflammation index (AEII) were determined in lung tissues. Results Culture supernatants and the BALF showed similar cytokine profiles. AA and GCs significantly inhibited HAT activity (P = 0.002 and P = 0.018), whereas TSA inhibited and GCs promoted HDAC activity (P = 0.004 and P = 0.025). HDACs 7, 9 and 10 were upregulated by AA and GCs (all P |
| Databáze: | OpenAIRE |
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